TheDose

Dehydroacetic Acid

Also known as Dehydroacetic acid, DHA, 3-Acetyl-6-methylpyran-2,4(3H)-dione, Methylacetopyranone

CIREU CosIngPubMed

Safe with conditions

EU CosIng says: restricted.”

Dehydroacetic acid (CAS 520-45-6) is a cyclic diketone preservative with fungicidal and bactericidal activity used in cosmetics at concentrations up to 0.6%. The CIR Expert Panel originally concluded it safe in 1985 (JACT 4(3):123-59) and reaffirmed that conclusion in 2003 and again in 2024 (Cherian et al., IJT 43(4 Suppl):130-134, PMID 38913451). A 2026 percutaneous absorption study confirmed limited skin penetration at permitted cosmetic concentrations (PMID 41666436). Toxicology studies using its sodium salt have identified potential anticoagulant effects via the mTOR/ERK pathway at systemic exposure levels (PMID 38103582), though these are not considered a concern at topical cosmetic use concentrations.


CIR Expert Panel has repeatedly concluded safe as used in cosmetic products (1985, 2003, 2024 re-reviews); no concentration cap imposed by CIR for leave-on or rinse-off applications

Effective antifungal preservative widely used in natural and conventional cosmetics at 0.1–0.6%; commonly paired with benzyl alcohol for broad-spectrum protection

Good stability across pH range typical for cosmetics; compatible with many formulation types including emulsions, shampoos, and leave-on creams

Permitted in organic/natural cosmetic certifications (COSMOS, ECOCERT) at permitted concentrations


Concerns
  • · EU Annex V explicitly prohibits use in aerosol dispensers (sprays) — inhalation risk is the implied rationale; products must not use this preservative in spray formats
  • · Limited antibacterial efficacy compared to antifungal potency — works primarily against fungi and yeasts with weaker action against bacteria, often used in combination with other preservatives

Anticoagulant effect mechanism identified for sodium dehydroacetate at systemic exposure levels via mTOR/ERK pathway — not a concern at topical cosmetic concentrations but flagged in precautionary toxicology literature

Regulatory discrepancy: EU Annex V entry number cited as Entry 33 in some references and Entry 13 in others — the entry covers the same chemical family (3-acetyl-6-methylpyran-2,4(3H)-dione and salts)


EU CosIng
Restricted (max 0.6%)
Use limit: 0.6%
Not to be used in aerosol dispensers (sprays). Max 0.6% as acid. Applies to all non-aerosol cosmetic products. (EU Annex V Entry 33)
CIR Expert Panel
Approved
[1]
CIR Expert Panel · Jul 1, 2018Archived

CIR Quick Reference Table (12/2017, revised 07/2018) — Dehydroacetic Acid row: Finding=S, Citation=JACT 4(3):123-59, 1985 confirmed 11/03…

Dehydroacetic Acid | S | | JACT 4(3):123-59, 1985 confirmed 11/03 IJT 25(S2), 2006QRT-122017revised072018.pdf, p. 42
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[2]
EU CosIng · Nov 30, 2009Document match

Regulation (EC) No 1223/2009 Annex V — Entry 33: 3-Acetyl-6-methylpyran-2,4(3H)-dione and its salts; max 0.6% (as acid); not to be used i…

Reference number 33: 3-Acetyl-6-methylpyran-2,4(3H)-dione and its salts -- 0,6 % (as acid); Not to be used in aerosol dispensers (sprays)Regulation (EC) No 1223/2009, Annex V, Entry 33
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[3]
Peer-reviewed (PubMed) · Jul 1, 2024

Sodium Dehydroacetate and Dehydroacetic Acid (Cherian P, Bergfeld WF, Belsito DV, et al.; Int J Toxicol 43(4 Suppl):130-134, 2024) — CIR …

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[4]
Peer-reviewed (PubMed) · Jan 1, 2026

In Vitro Percutaneous Absorption of Dehydroacetic Acid and Benzoic Acid From Pig Skin Using the Franz Diffusion Cell System (Chung HN, Ki…

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[5]
Peer-reviewed (PubMed) · Jan 1, 2024

The mechanism of blood coagulation induced by sodium dehydroacetate via the regulation of the mTOR/ERK pathway in rats (Zhang M, Zhang Q,…

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Sources
5
PubMed citations
3
Evidence quality
moderate
Last verified
Re-reviewed when a new CIR / SCCS opinion publishes.